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Overview of LMNOP-bot. Nitrogen tank supplies pressure to four pressure <t>regulators,</t> which recieve the set pressure, P s (t), from a microcontroller, and return the measured pressure, P m (t). Pressures P 1:4, m enter component reservoirs C 1:4 . Tubing leaving the reservoirs contributes resistances R 1:4,a , while microfluidic channels in a PDMS chip contribute further resistances R 1:4,b , and the exit channel and outlet needle contribute shared resistance R 5 . The chip is sandwiched between two layers of a simple chip holder which screws into an acrylic top plate. The microcontroller actuates motion in X, Y, and Z directions in order to collect product from the chip into a well plate.
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Overview of LMNOP-bot. Nitrogen tank supplies pressure to four pressure <t>regulators,</t> which recieve the set pressure, P s (t), from a microcontroller, and return the measured pressure, P m (t). Pressures P 1:4, m enter component reservoirs C 1:4 . Tubing leaving the reservoirs contributes resistances R 1:4,a , while microfluidic channels in a PDMS chip contribute further resistances R 1:4,b , and the exit channel and outlet needle contribute shared resistance R 5 . The chip is sandwiched between two layers of a simple chip holder which screws into an acrylic top plate. The microcontroller actuates motion in X, Y, and Z directions in order to collect product from the chip into a well plate.
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Overview of LMNOP-bot. Nitrogen tank supplies pressure to four pressure regulators, which recieve the set pressure, P s (t), from a microcontroller, and return the measured pressure, P m (t). Pressures P 1:4, m enter component reservoirs C 1:4 . Tubing leaving the reservoirs contributes resistances R 1:4,a , while microfluidic channels in a PDMS chip contribute further resistances R 1:4,b , and the exit channel and outlet needle contribute shared resistance R 5 . The chip is sandwiched between two layers of a simple chip holder which screws into an acrylic top plate. The microcontroller actuates motion in X, Y, and Z directions in order to collect product from the chip into a well plate.

Journal: bioRxiv

Article Title: Open-source robotic chip-to-plate interface for high-throughput microfluidic generation of materials libraries

doi: 10.64898/2026.05.12.724546

Figure Lengend Snippet: Overview of LMNOP-bot. Nitrogen tank supplies pressure to four pressure regulators, which recieve the set pressure, P s (t), from a microcontroller, and return the measured pressure, P m (t). Pressures P 1:4, m enter component reservoirs C 1:4 . Tubing leaving the reservoirs contributes resistances R 1:4,a , while microfluidic channels in a PDMS chip contribute further resistances R 1:4,b , and the exit channel and outlet needle contribute shared resistance R 5 . The chip is sandwiched between two layers of a simple chip holder which screws into an acrylic top plate. The microcontroller actuates motion in X, Y, and Z directions in order to collect product from the chip into a well plate.

Article Snippet: LMNOP-bot is controlled by an Arduino microcontroller (Arduino MEGA 2560 Rev3, Arduino, Italy), which synchronizes changes in the set pressures, P s , measured pressures, P m , and plate location ( ) through the pressure regulators (PCD-30PSIG-D-PCV30/5P, RIN, 5IN, RANGE (50 PSIG) P1: 100 PSIG, P2: ATM, VOL: 15 mL, Alicat, USA) and motor drivers (A4988 Stepper Motor Driver Carrier, #1182, Pololu, USA).

Techniques:

Control of synchronized motion and pressure. Arduino code is used to set the collection plate layout in terms of rows, columns, and spacing, the number of component inputs, the timing and tolerance preferences, and the desired pressure combinations. Once started, the Arduino controls both stepper motors and pressure regulators in order to generate and collect the desired formulations.

Journal: bioRxiv

Article Title: Open-source robotic chip-to-plate interface for high-throughput microfluidic generation of materials libraries

doi: 10.64898/2026.05.12.724546

Figure Lengend Snippet: Control of synchronized motion and pressure. Arduino code is used to set the collection plate layout in terms of rows, columns, and spacing, the number of component inputs, the timing and tolerance preferences, and the desired pressure combinations. Once started, the Arduino controls both stepper motors and pressure regulators in order to generate and collect the desired formulations.

Article Snippet: LMNOP-bot is controlled by an Arduino microcontroller (Arduino MEGA 2560 Rev3, Arduino, Italy), which synchronizes changes in the set pressures, P s , measured pressures, P m , and plate location ( ) through the pressure regulators (PCD-30PSIG-D-PCV30/5P, RIN, 5IN, RANGE (50 PSIG) P1: 100 PSIG, P2: ATM, VOL: 15 mL, Alicat, USA) and motor drivers (A4988 Stepper Motor Driver Carrier, #1182, Pololu, USA).

Techniques: Control