Journal: bioRxiv
Article Title: Open-source robotic chip-to-plate interface for high-throughput microfluidic generation of materials libraries
doi: 10.64898/2026.05.12.724546
Figure Lengend Snippet: Overview of LMNOP-bot. Nitrogen tank supplies pressure to four pressure regulators, which recieve the set pressure, P s (t), from a microcontroller, and return the measured pressure, P m (t). Pressures P 1:4, m enter component reservoirs C 1:4 . Tubing leaving the reservoirs contributes resistances R 1:4,a , while microfluidic channels in a PDMS chip contribute further resistances R 1:4,b , and the exit channel and outlet needle contribute shared resistance R 5 . The chip is sandwiched between two layers of a simple chip holder which screws into an acrylic top plate. The microcontroller actuates motion in X, Y, and Z directions in order to collect product from the chip into a well plate.
Article Snippet: LMNOP-bot is controlled by an Arduino microcontroller (Arduino MEGA 2560 Rev3, Arduino, Italy), which synchronizes changes in the set pressures, P s , measured pressures, P m , and plate location ( ) through the pressure regulators (PCD-30PSIG-D-PCV30/5P, RIN, 5IN, RANGE (50 PSIG) P1: 100 PSIG, P2: ATM, VOL: 15 mL, Alicat, USA) and motor drivers (A4988 Stepper Motor Driver Carrier, #1182, Pololu, USA).
Techniques: